开户送58体验金官网

2023.10 ：卣庞詈苹竦2023年硕士研究生国家奖学金！

2023.5 ：鼗剖锘莼竦2023年开户送58体验金官网研究生 (博士) 优秀毕业生称号！

2023.5 李健受邀参加第52期 SATOL 科学Talk "合成生物，创启未来的造物方式"，并做以“无细胞合成生物学”为主题的讲座。

2023.5 课题组参加第八届上：铣缮镅Т葱抡铰粤四昊，李健主持新发展论坛一“合成生物学源头创新”；巴方和季向阳同学分别获得“优秀海报奖”！

2023.5 课题组关于无细胞生物合成非天然氨基酸的研究论文入选美国化学会 ACS Editors' Choice 亮点报道！学校新闻

2023.4 课题组关于构建大肠杆菌利用赤藓糖醇的研究成果在Advanced Science期刊发表，并受到多家媒体及公众号的关注与报道！学校新闻 (中文)、学校新闻 (英文)、科学网、中国科学报、生辉SynBio

2023.1 李健受邀在Nature Chemistry发表 “新闻&观点 (News&Views) ” 文章。文章链接

2022.4 课题组关于SYMBIOSIS的论文被Faculty Opinions (原F1000 Prime) 推荐介绍，并被推荐为最高等级 (Exceptional)，推荐全文如下：

2022.3 李健受邀担任Frontiers in Natural Products杂志创刊副主编 (Associate Editor)，负责天然产物生物合成方向稿件。

2021.9 李健受邀继续担任Frontiers in Bioengineering and Biotechnology 客座编辑（Guest Topic Editor）出版以“Cell-Free Synthetic Biology, Volume II”为主题的第二期研究专刊（Research Topic）。https://www.frontiersin.org/research-topics/26520/cell-free-synthetic-biology-volume-ii

2021.7 实验室组织多人参加中国生物工程学会第五届青年科技论坛，李健做题为《无细胞合成生物学驱动高效生物合成》的会议报告。

2021.5 实验室组织多人参加第四届生物化工青年学者论坛，大家通过参会广泛了解研究进展，并与学术同行进行了深入交流，扩展了大家的视野。

2021.4 实验室组织前往上海迪士尼春游活动，学习之余大家放松了身心。

2021.3 李健参加德国第六届BioProScale在线会议并做Poster报告，同时获得会议Poster奖 (2nd Place Award)！

2021.3 课题组邀请清华大学卢元副教授到实验室交流，并做题为《无细胞合成生物学：从原型设计到应用拓展》的学术报告。

2021.1 课题组举行2020年年终总结交流会，大家总结了过去，展望了未来，对新的一年充满了憧憬！

2020.12 实验室组织多人参加2020年上海市微生物学会/生物工程学会青年论坛，李健受邀做会议报告，许慧玲获得论坛“优秀墙报”奖！

2020.10 实验室组织农家乐秋游活动，挖红薯、垂钓、烧烤、聚餐...大家通过这次活动放松了身心，增进了交流！

2019.12 课题组邀请上海交通大学林双君教授到实验室交流，并做题为《微生物生物碱生物合成》的学术报告。

2019.11 课题组邀请中科院上海生命科学研究院植物生理生态研究所王勇研究员到实验室交流，并做题为Natural Product Synthetic Biology: From Bacteria to Plant的学术报告。

2019.10 李健受邀担任Frontiers in Bioengineering and Biotechnology 客座编辑（Guest Topic Editor）出版以“Cell-Free Synthetic Biology”为主题的研究专刊（Research Topic）。https://www.frontiersin.org/research-topics/12103/cell-free-synthetic-biology

2019.9 课题组邀请复旦大学张琪教授到实验室交流，并做题为Discovery and Biosynthesis of Ribosomally Synthesized and Posttranslationally Modified Peptides (RiPPs)的学术报告。

2019.4 李健参加中国生物工程学会第四届青年科技论坛并做大会报告，会前，李健入选为中国生物工程学会青年工作委员会委员。

2019.4 实验室组织前往上海野生动物园一日游，科研之余，大家放松了心情，加强了交流，增进了感情！

2019.3 李健受邀参加由日本生物工学会主办的首届Sakura-Bio会议并做大会报告。

2018.10 李健担任共同指导老师带领开户送58体验金官网iGEM团队获得2018年国际基因工程机器大赛（International Genetically Engineered Machine Competition, iGEM）全球总决赛金牌。

2018.8 李健受邀担任Springer Nature出版社《SN Applied Sciences》杂志编委。

2018.7 李健参加第四届合成生物学青年学者论坛（Synthetic Biology Young Scholar Forum）并做大会报告。

2017.9 开户送58体验金官网无细胞合成生物学（Cell-Free Synthetic Biology）课题组正式成立！

52. Lu H, Ouyang J, Liu WQ, Wu C*, Li J*. Enzyme-polymer-conjugate-based Pickering emulsions for cell-free expression and cascade biotransformation. Angewandte Chemie International Edition, 2023, e202312906 

51. Ba F, Zhang Y, Ji X, Liu WQ, Ling S, Li J*. Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology. Biotechnology Journal, 2023, 2300327 

50. Zhang Y, Liu WQ, Li J*. Constructing an artificial short route for cell-free biosynthesis of the phenethylisoquinoline scaffold. Synthetic and Systems Biotechnology, 2023, 8, 610–617

49. Wang Y, Gao W, Yang S, Chen Q, Ye C, Wang H, Zhang Q, Ren J, Ning Z, Chen X, Shao Z, Li J, Liu Y, Ling S. Humanoid intelligent display platform for audiovisual interaction and sound identification. Nano-Micro Letters, 2023, 15, 221

48. Shi Q, Li J, Liu C, Zhai X, Chen L, Zhang Y, Feng D, Zhang R, Li J, Ling S, Zheng L, Luo Y, Liu Y. Fluorescence-coded logarithmic-dilution digital droplet PCR for ultrawide-dynamic-range nucleic acid quantification. Biosensors and Bioelectronics, 2023, 241, 115702

47. Ba F, Zhang Y, Wang L, Liu WQ, Li J*. Applications of serine integrases in synthetic biology over the past decade. SynBio, 2023, 1, 172–189

46.  Peng R#, Ba F#, Li J#, Cao J, Zhang R, Liu WQ, Ren J, Liu Y*, Li J*, Ling S*. Embedding living cells with a mechanically reinforced and functionally programmable hydrogel fiber platform. Advanced Materials, 2023, 35, 2305583 新闻报道

45. Dong Z, Peng R, Zhang Y, Shan Y, Ding W, Liu Y, Li J, Zhao M, Jiang LB, Ling S. Tendon repair and regeneration using bioinspired fibrillation engineering that mimicked the structure and mechanics of natural tissue. ACS Nano, 2023, 17, 17858–17872

44. Zhu J, Dai C, Yang H, Shan Y, Ren J, Liu Y, Li J, Chen W, Liang J, Ling S. IntelliSense bio-ionotronics battery for early warning of geological seepage. ACS Sensors, 2023, 8, 2731–2739

43.  Zhang R, Liu WQ, Ling S, Li J*. Combining cell-free expression and multifactor optimization for enhanced biosynthesis of cinnamyl alcohol. Journal of Agricultural and Food Chemistry, 2023, 71, 8551–8557

42. Chen Y, Liu WQ, Zheng X, Liu Y, Ling S, Li J*. Cell-free biosynthesis of lysine-derived unnatural amino acids with chloro, alkene, and alkyne groups. ACS Synthetic Biology, 2023, 12, 1349–1357  (Highlighted as ACS Editors' Choice on May 4, 2023) 新闻报道

41. Meng Y, Yang M, Liu WQ, Li J*. Cell-free expression of a therapeutic protein serratiopeptidase. Molecules, 2023, 28, 3132

40. Ba F, Ji X, Huang S, Zhang Y, Liu WQ, Liu Y, Ling S, Li J*. Engineering Escherichia coli to utilize erythritol as sole carbon source. Advanced Science, 2023, 10, 2207008 新闻报道

39. Zhang H, Cao L, Li J, Liu Y, Lv Z, Ren J, Shao Z, Ling S. Dual physically crosslinked silk fibroin ionoelastomer with ultrahigh stretchability and low hysteresis. Chemistry of Materials, 2023, 35, 1752–1761

38. Huang S, Ba F, Liu WQ, Li J*. Stapled NRPS enhances the production of valinomycin in Escherichia coli. Biotechnology and Bioengineering, 2023, 120, 793–802

37. Liu Y, Liu WQ, Huang S, Xu H, Lu H, Wu C, Li J*. Cell-free metabolic engineering enables selective biotransformation of fatty acids to value-added chemicals. Metabolic Engineering Communications, 2023, 16, e00217

36.  Liu WQ, Ji X, Xu H, Lu Y, Li J*. Cell-free protein synthesis system enables rapid and efficient biosynthesis of restriction endonucleases. Synthetic Biology Journal (in Chinese), 2023, 4, 840–851

刘晚秋，季向阳，许慧玲，卢屹聪，李健*. 限制性内切酶的无细胞快速制备研究. 合成生物学, 2023, 4, 840–851

35. Li J*. Cellular decoding for non-natural peptides. Nature Chemistry, 2023, 15, 7–8

34. Moore SJ, Lai HE,  Li J, Freemont PS. Streptomyces cell-free systems for natrual product discovery and engineering. Natural Product Reports, 2023, 40, 228–236

33. Li L, Zhang R, Chen L, Tian X, Li T, Pu B, Ma C, Ji X, Ba F, Xiong C, Shi Y, Mi X, Li J*, Keasling JD, Zhang J*, Liu Y*. Permeability-engineered compartmentation enables in vitro reconstitution of sustained synthetic biology systems. Advanced Science, 2022, 2203652

32. Yang C#, Yang M#, Zhao W, Ding Y, Wang Y*, Li J*. Establishing a Klebsiella pneumoniae-based cell-free protein synthesis system. Molecules, 2022, 27, 4684

31. Sun Z, Hübner R, Li J, Wu C. Artificially sporulated Escherichia coli cells as a robust cell factory for interfacial biocatalysis. Nature Communications, 2022, 13, 3142

30. Zhang Y, Chen W, Wu D, Liu Y, Wu Z, Li J, Zhang SY, Ji Q. Molecular basis for cell-wall recycling regulation by transcriptional repressor MurR in Escherichia coli. Nucleic Acids Research, 2022, 50, 5948–5960

29. Li J*. Engineered microbes: Making valuable chemicals from waste gases. Synthetic and Systems Biotechnology, 2022, 7, 837–838

28. Tian X, Liu WQ, Xu H, Ji X, Liu Y, Li J*. Cell-free expression of NO synthase and P450 enzyme for the biosynthesis of an unnatural amino acid L-4-nitrotryptophan. Synthetic and Systems Biotechnology, 2022, 7, 775–783

27. Liu Y, Ba F, Liu WQ, Wu C, Li J*. Plug-and-play functionalization of protein-polymer conjugates for tunable catalysis enabled by genetically encoded "click" chemistry. ACS Catalysis, 2022, 12, 4165–4174 新闻报道

26. Ji X#, Liu WQ#, Li J*. Recent advances in applying cell-free systems for high-value and complex natural product biosynthesis. Current Opinion in Microbiology, 2022, 67, 102142 (Invited review)

25. Ba F, Liu Y, Liu WQ, Tian X, Li J*. SYMBIOSIS: Synthetic manipulable biobricks via orthogonal serine integrase systems. Nucleic Acids Research, 2022, 50, 2973–2985 (Faculty Opinions Recommended as Exceptional) 新闻报道

24. Xu H, Yang C, Tian X, Chen Y, Liu WQ, Li J*. Regulatory part engineering for high-yield protein synthesis in an all-Streptomyces-based cell-free expression system. ACS Synthetic Biology, 2022, 11, 570–578

23. Xu H, Liu WQ, Li J*. A Streptomyces-based cell-free protein synthesis system for high-level protein expression. Methods in Molecular Biology, 2022, 2433, 89–103

22. Li J*, Kwon YC*, Lu Y*, Moore SJ*. Editorial: Cell-free synthetic biology. Frontiers in Bioengineering and Biotechnology, 2021, 9, 799122

21. Yang C, Liu Y, Liu WQ, Wu C, Li J*. Designing modular cell-free systems for tunable biotransformation of L-phenylalanine to aromatic compounds. Frontiers in Bioengineering and Biotechnology, 2021, 9, 730663

20. Huang S#, Liu Y#, Liu WQ, Neubauer P*, Li J*. The nonribosomal peptide valinomycin: From discovery to bioactivity and biosynthesis. Microorganisms, 2021, 9, 780 (Highly Cited Paper)

19. Feng J#, Yang C#, Zhao Z, Xu J, Li J*, Li P*. Application of cell-free protein synthesis system for the biosynthesis of L-theanine. ACS Synthetic Biology, 2021, 10, 620–631

18. Liu WQ, Wu C*, Jewett MC, Li J*. Cell-free protein synthesis enables one-pot cascade biotransformation in an aqueous-organic biphasic system. Biotechnology and Bioengineering, 2020, 117, 4001–4008

17. Zhang L, Liu WQ, Li J*. Establishing a eukaryotic Pichia pastoris cell-free protein synthesis system. Frontiers in Bioengineering and Biotechnology, 2020, 8, 536 新闻报道

16. Xu H, Liu WQ, Li J*. Translation related factors improve the productivity of a Streptomyces-based cell-free protein synthesis system. ACS Synthetic Biology, 2020, 9, 1221–1224 新闻报道

15. Zhuang L#, Huang S#, Liu WQ, Karim AS, Jewett MC*, Li J*. Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin. Metabolic Engineering, 2020, 60, 37–44 新闻报道

14. Liu WQ#, Zhang L#, Chen M#, Li J*. Cell-free protein synthesis: Recent advances in bacterial extract sources and expanded applications. Biochemical Engineering Journal, 2019, 141, 182–189

13. Li J*, Zhang L, Liu WQ. Cell-free synthetic biology for in vitro biosynthesis of pharmaceutical natural products. Synthetic and Systems Biotechnology, 2018, 3, 83–89

12.Wang H, Li J*, Jewett MC*. Development of a Pseudomonas putida cell-free protein synthesis platform for rapid screening of gene regulatory elements. Synthetic Biology, 2018, 3, ysy003

PhD and Postdoc publications

11.Casini A, Chang FY, Eluere R, King A, Young EM, Dudley QM, Karim A, Pratt K, Bristol C, Forget A, Ghodasara A, Warden-Rothman R, Gan R, Cristofaro A, Espah Borujeni A, Ryu MH, Li J, Kwon YC, Wang H, Tatsis E, Rodriguez-Lopez C, O'Connor S, Medema MH, Fischbach M, Jewett MC, Voigt CA, Gordon DB. A pressure test to make 10 molecules in 90 days: external evaluation of methods to engineer biology. Journal of the American Chemical Society, 2018, 140, 4302–4316

10. Li J, Wang H, Jewett MC. Expanding the palette of Streptomyces-based cell-free protein synthesis systems with enhanced yields. Biochemical Engineering Journal, 2018, 130, 29–33

9. Li J, Wang H, Kwon YC, Jewett MC. Establishing a high yielding Streptomyces-based cell-free protein synthesis system. Biotechnology and Bioengineering, 2017, 114, 1343–1353 (Video Highlighted)

8. Goering AW#, Li J#, McClure RA, Thomson RJ, Jewett MC, Kelleher NL. In vitro reconstruction of nonribosomal peptide biosynthesis directly from DNA using cell-free protein synthesis. ACS Synthetic Biology, 2017, 6, 39–44

7. Li J, Lawton TJ, Kostecki JS, Nisthal A, Fang J, Mayo SL, Rosenzweig AC, Jewett MC. Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnology Journal, 2016, 11, 212–218 (Front Cover)

6. Moatsou D#, Li J#, Ranji A, Pitto-Barry A, Ntai I, Jewett MC, O’Reilly RK. Self-assembly of temperature-responsive protein-polymer bioconjugates. Bioconjugate Chemistry, 2015, 26, 1890–1899 (Front Cover)

5. Li J*, Jaitzig J, Lu P, Süssmuth RD, Neubauer P. Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations. Microbial Cell Factories, 2015, 14, 83

4. Li J*, Jaitzig J, Theuer L, Legala OE, Süssmuth RD, Neubauer P. Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli. Journal of Biotechnology, 2015, 193, 16–22

3. Li J*, Neubauer P. Escherichia coli as a cell factory for heterologous production of nonribosomal peptides and polyketides. New Biotechnology, 2014, 31, 579–585

2. Li J#, Jaitzig J#, Hillig F, Süssmuth RD, Neubauer P. Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation. Applied Microbiology and Biotechnology, 2014, 98, 591–601

1. Jaitzig J#, Li J#, Süssmuth RD, Neubauer P. Reconstituted biosynthesis of the nonribosomal macrolactone antibiotic valinomycin in Escherichia coli. ACS Synthetic Biology, 2014, 3, 432–438